AgTech: Soybean Rust-Resistant Plant
It took decades of painstaking work, but research geneticist Ram Singh managed to cross a popular soybean variety ("Dwight" Glycine max) with a related wild perennial plant that grows like a weed in Australia, producing the first fertile soybean plants that are resistant to soybean rust, soybean cyst nematode and other pathogens of soy.
Singh works in the Soybean/Maize Germplasm, Pathology and Genetics Research unit in the department of crop sciences at the University of Illinois at Urbana-Champaign. The unit is a division of the U.S. Department of Agriculture's Agricultural Research Program.
His efforts to introduce the desirable attributes of wild, perennial Glycine species into soybean plants began at the U. of I. in 1983 and followed a path that involved thousands of experiments, the development of a hormone treatment that "rescued" immature hybrid seeds from sterility, and multiple back-crosses of hybrid plants with their "recurrent parent," Dwight.
Singh's collaborator, Randall Nelson, the research leader of the ARS soybean/maize research unit, plants seeds from Singh's most promising experiments, grows the plants and distributes their seeds to other scientists, who screen them for desirable traits and conduct their own breeding experiments.
"There are 26 wild species of Glycine perennials that grow in Australia," he said. One species, Glycine tomentella, was of particular interest because it has genes for resistance to soybean rust and to soybean cyst nematode, he said. "Many people tried to hybridize it with soybean plants, starting back in 1979 at the University of Illinois." But the hybrids produced only sterile plants, "and they decided it was impossible," Singh said.
He continued to experiment, however, and eventually developed a hormone treatment that interrupted the process that caused the hybrid seeds to abort. He also developed a tissue culture method for producing several embryos - and thus, several plants - from each seed. The plants were grown in a greenhouse, allowed to flower and crossed again with Dwight.
Singh eventually settled on a Glycine tomentella plant known as PI 441001 for these experiments because the wild plant was immune to soybean rust and to soybean cyst nematode. It also was resistant to Phytophthora root rot and could tolerate salt and drought.
Source : http://www.eurekalert.org/
Singh works in the Soybean/Maize Germplasm, Pathology and Genetics Research unit in the department of crop sciences at the University of Illinois at Urbana-Champaign. The unit is a division of the U.S. Department of Agriculture's Agricultural Research Program.
His efforts to introduce the desirable attributes of wild, perennial Glycine species into soybean plants began at the U. of I. in 1983 and followed a path that involved thousands of experiments, the development of a hormone treatment that "rescued" immature hybrid seeds from sterility, and multiple back-crosses of hybrid plants with their "recurrent parent," Dwight.
Singh's collaborator, Randall Nelson, the research leader of the ARS soybean/maize research unit, plants seeds from Singh's most promising experiments, grows the plants and distributes their seeds to other scientists, who screen them for desirable traits and conduct their own breeding experiments.
"There are 26 wild species of Glycine perennials that grow in Australia," he said. One species, Glycine tomentella, was of particular interest because it has genes for resistance to soybean rust and to soybean cyst nematode, he said. "Many people tried to hybridize it with soybean plants, starting back in 1979 at the University of Illinois." But the hybrids produced only sterile plants, "and they decided it was impossible," Singh said.
He continued to experiment, however, and eventually developed a hormone treatment that interrupted the process that caused the hybrid seeds to abort. He also developed a tissue culture method for producing several embryos - and thus, several plants - from each seed. The plants were grown in a greenhouse, allowed to flower and crossed again with Dwight.
Singh eventually settled on a Glycine tomentella plant known as PI 441001 for these experiments because the wild plant was immune to soybean rust and to soybean cyst nematode. It also was resistant to Phytophthora root rot and could tolerate salt and drought.
Source : http://www.eurekalert.org/